Recombinant ERa Antibody / Estrogen Receptor alpha

The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P03372

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant protein fragment from the C-terminal region was used as the immunogen for the recombinant ERa antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ESR1/4039R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant ERa antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant ERa antibody is available for research use only.

Storage Conditions

Aliquot the recombinant ERa antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant ERa antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human breast carcinoma tissue with recombinant ERa antibody (clone ESR1/4039R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.