AGO3 Antibody / Argonaute-3 / eIF2C3
This antigen is involved in binding and translation repression of short RNAs. This antigen is also known as Argonaute RISC catalytic component 3. Required for RNA-mediated gene silencing (RNAi) . Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5-flanking sequences to the region of guide-target complementarity.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
Q9H9G7
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human AGO3 protein was used as the immunogen for the AGO3 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
PCRP-AGO3-1C5
Applications
ELISA, FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the AGO3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This AGO3 antibody is available for research use only.
Storage Conditions
Aliquot the AGO3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the AGO3 antibody should be determined by the researcher.
Location
Cytoplasm, Nucleus
Image Legend
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