CD23 Antibody

CD23 (FCE2) is a type II integral membrane glycoprotein that is expressed on mature B cells, monocytes, eosinophils, platelets and dendritic cells. CD23 is a low affinity IgE receptor that mediates IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P06734

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 48-321 was used as the immunogen for the CD23 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

FCER2/6887

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CD23 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD23 antibody is available for research use only.

Storage Conditions

Aliquot the CD23 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD23 antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human tonsil tissue with CD23 antibody (clone FCER2/6887) at 2ug/ml in PBS for 30min RT. Membrane staining observed. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.