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58K Golgi protein Antibody / Golgi Marker

The antibody recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein. It is also useful for studies on the effect of microtubule-perturbing agents on the Golgi apparatus.The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively-spliced transcript variants have been found for this gene. Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool. Binds and promotes bundling of vimentin filaments originating from the Golgi.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

O95954

Host

Mouse

Reactivity

Human

Immunogen

58K Golgi protein purified from rat liver was used as the immunogen for the 58K Golgi protein antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

FTCD/357

Applications

FACS, IF, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the 58K Golgi protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This 58K Golgi protein antibody is available for research use only.

Storage Conditions

Aliquot the 58K Golgi protein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the 58K Golgi protein antibody should be determined by the researcher.

Location

Cytoplasm, Golgi apparatus

Image Legend

SDS-PAGE analysis of purified, BSA-free 58K Golgi protein antibody (clone FTCD/357) as confirmation of integrity and purity.

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