IDH1 Antibody / Isocitrate Dehydrogenase (R132H mutant)

IDH1 R132H antibody binds to IDH1-mutated protein, but does not bind the wild-type IDH1 protein. IDH1 R132H point mutations are frequently seen in World Health Organization grade II and III gliomas and are believed to constitute an early step in tumorigenesis. IDH1 R132H can be used as a diagnostic marker to help differentiate infiltrating gliomas from gliosis, and as a prognostic marker for gliomas and secondary glioblastoma multiforme. IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide. Diffuse staining of the fibrillary tumor matrix is also seen.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

O75874

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 281-414 was used as the immunogen for the IDH1 R132H antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

IDH1/6806R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the IDH1 R132H antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This IDH1 R132H antibody is available for research use only.

Storage Conditions

Aliquot the IDH1 R132H antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the IDH1 R132H antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of IDH1-R132H mutation protein in FFPE human prostate carcinoma tissue with IDH1-R132H antibody (clone IDH1/6806R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.