Oct4 Antibody / POU5F1
Oct4 Transcription factor that binds to the octamer motif (5) . Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
Q01860
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 1-134 was used as the immunogen for the Oct4. antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
Oct4/3508
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Oct4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Oct4 antibody is available for research use only.
Storage Conditions
Aliquot the Oct4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Oct4 antibody should be determined by the researcher.
Location
Nucleus
Image Legend
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