Recombinant GATA3 Antibody

GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

P23771

Host

Rabbit

Reactivity

Human, Rat

Immunogen

A conserved peptide between the GATA trans-activation and DNA-binding domain was used as the immunogen for the recombinant GATA3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

GATA3/4550R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant GATA3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant GATA3 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant GATA3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant GATA3 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of human MCF7 cell lysate with recombinant GATA3 antibody (clone GATA3/4550R) . Observed molecular weight: ~50 kDa (full length), ~37 kDa (splice form) .