Biotin Antibody
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL
UniProt
Not Applicable
Host
Mouse
Reactivity
All Species Expected
Immunogen
Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
BTN/403
Applications
FACS, IF, WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Biotin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Biotin antibody is available for research use only.
Storage Conditions
Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/biotin-antibody-btn403-v9363
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Biotin antibody should be determined by the researcher.
Image Legend
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
HSP60 (Heat Shock Protein 60, 60kD chaperonin, 60kD heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic para
HSP60 (Heat Shock Protein 60, 60kD chaperonin, 60kD heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic para
![Mouse Monoclonal Complement Component C3aR Antibody (HC3aRZ8) [Alexa Fluor 405]](https://cdn.genprice.uk/genprice/genprice5.png)
Mouse Monoclonal Complement Component C3aR Antibody (HC3aRZ8) [Alexa Fluor 405]