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Recombinant hCG alpha Antibody

Human chorionic gonadotropin antibody (hCG) is a glycoprotein hormone synthesized in syncytiotrophoblastic cells of placenta and in certain trophoblastic tumors. The hormone-specific alpha chains have molecular weights of 13 kDa. HCG is found in moles and choriocarcinoma, chorionic components of germ cell tumors, and syncytiotrophoblast like cells in seminoma/dysgerminoma and embryonal carcinoma. In diagnostic pathology, hCG is a useful marker for classification of germ cell tumors, identification of extragonadal germ cell tumors.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P01215

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-100 of hCG protein was used as the immunogen for the recombinant hCG alpha antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

HCGa/2728R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant hCG alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant hCG alpha antibody is available for research use only.

Storage Conditions

Aliquot the recombinant hCG alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant hCG alpha antibody should be determined by the researcher.

Location

Cytoplasmic, secreted

Image Legend

IHC staining of FFPE human placental tissue with recombinant hCG alpha antibody (clone HCGa/2728R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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