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Recombinant MPO Antibody / Myeloperoxidase

Myeloperoxidase (MPO) also called the peroxidase (POD), is an important marker of bone marrow cells. It is one of the members of the family of heme peroxidase super existing in myeloid cells (mainly neutrophils and monocytes of aniline blue particles) . With the deepening of the research on MPO, MPO gene polymorphism has been found to lead to individual for some disease susceptibility differences, with a variety of human development is closely related to the occurrence of diseases. The antibody reacts with neutrophil granulocytes and monocytes in blood and with precursors of granulocytes in the bone marrow. The antibody is useful as an aid for classification of neoplastic tissue, i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

P05164

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 150-250 was used as the immunogen for the recombinant MPO antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RMPO/6904

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant MPO antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant MPO antibody is available for research use only.

Storage Conditions

Aliquot the recombinant MPO antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant MPO antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human bone marrow with recombinant MPO antibody (clone rMPO/6904) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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