Recombinant Keratin 8 Antibody / Cytokeratin 8

Cytokeratin 8 (also called CK8, Keratin 8 and KRT8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18) . CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P05787

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human KRT8 protein was used as the immunogen for the recombinant Keratin 8 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

KRT8/6472R

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant Keratin 8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Keratin 8 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Keratin 8 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant Keratin 8 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human colon tissue with recombinant Keratin 8 antibody (clone KRT8/6472R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.