PDGF beta Antibody

PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms; PDGF AA, AB and BB. The three isoforms bind, with different affinities, to two receptor types, receptors. Evidence suggests that PDGF may function as a neurotrophic factor. Receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons. These facts suggest that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system through paracrine and autocrine routes.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P01127

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 27-158 was used as the immunogen for the PDGF beta antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

PDGFB/3071

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the PDGF beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PDGF beta antibody is available for research use only.

Storage Conditions

Aliquot the PDGF beta antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the PDGF beta antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using PDGF beta antibody (clone PDGFB/3071) . These results demonstrate the foremost specificity of the PDGFB/3071 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.