CD48 Antibody (Pan Leukocyte Marker)

Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI) -anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P09326

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 59-178 was used as the immunogen for the CD48 antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

CD48/4785

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the CD48 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD48 antibody is available for research use only.

Storage Conditions

Aliquot the CD48 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the CD48 antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human lymph node with CD48 antibody (clone CD48/4785) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.