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Recombinant Topoisomerase II alpha Antibody / TOP2A

The topoisomerase IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P11388

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1352-1493 was used as the immunogen for the recombinant Topoisomerase II alpha antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

TOP2A/6570R

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Topoisomerase II alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Topoisomerase II alpha antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Topoisomerase II alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Topoisomerase II alpha antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human lymph node tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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