Recombinant Basic Cytokeratin Antibody (HMW / Type II)

The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q01546

Host

Mouse

Reactivity

Human

Immunogen

A synthetic peptide sequence common to Type II Cytokeratins was used as the immunogen for the recombinant Basic Cytokeratin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RKRTH/6617

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant Basic Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Basic Cytokeratin antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Basic Cytokeratin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human skin with recombinant Basic Cytokeratin antibody (clone rKRTH/6617) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.