Recombinant TOP2A Antibody / Topoisomerase II alpha

It recognizes a 170kDa protein, which is identified as topoisomerase II is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL

UniProt

P11388

Host

Mouse

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 1352-1493 was used as the immunogen for the recombinant TOP2A antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2a κ

Clone

RTOP2A/6569

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant TOP2A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant TOP2A antibody is available for research use only.

Storage Conditions

Aliquot the recombinant TOP2A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human lymph node with recombinant TOP2A antibody (clone rTOP2A/6569) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.