Recombinant Acidic Cytokeratin Antibody (LMW / Type I)

The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. Its usually used in a cytokeratin cocktail with type II cytokeratin antibody.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q7Z794

Host

Mouse

Reactivity

Human

Immunogen

A synthetic peptide sequence common to Type I Cytokeratins was used as the immunogen for the recombinant Acidic Cytokeratin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RKRTL/6616

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Acidic Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Acidic Cytokeratin antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Acidic Cytokeratin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Acidic Cytokeratin antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human sebaceous gland with recombinant Acidic Cytokeratin antibody (clone rKRTL/6616) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.