Recombinant MSH2 Antibody

Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) . Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

IDP43246

Host

Mouse

Reactivity

Human, Cat, Dog

Immunogen

Recombinant full-length human MSH2 protein was used as the immunogen for the recombinant MSH2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2a κ

Clone

RMSH2/6548

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant MSH2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant MSH2 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant MSH2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant MSH2 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human colon tissue with recombinant MSH2 antibody (clone rMSH2/6548) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.