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PGP9.5 / UchL1 Antibody

PGP9.5 / UchL1 is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P09936

Host

Mouse

Reactivity

Human, Rat

Immunogen

KLH conjugated synthetic peptide selected from the 185-214 region of human UCHL1 was used as the immunogen for the PGP9.5 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

UCHL1/4558

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the PGP9.5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PGP9.5 antibody is available for research use only.

Storage Conditions

Aliquot the PGP9.5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the PGP9.5 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using PGP9.5 antibody (clone UCHL1/4558) . These results demonstrate the foremost specificity of the UCHL1/4558 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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