Smooth Muscle Actin Antibody
Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells.</strong> This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P62736
Host
Mouse
Reactivity
Human
Immunogen
N-Terminal decapeptide of alpha smooth muscle isoform of actin was used as the immunogen for the Smooth Muscle Actin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RACTA2/1625
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Smooth Muscle Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Smooth Muscle Actin antibody is available for research use only.
Storage Conditions
Aliquot the Smooth Muscle Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Smooth Muscle Actin antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
IL-10R1, Mouse (Interleukin-10 Receptor 1) (CD210) (1B1.3a), CF405S conjugate, 0.1mg/mL
