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LEF1 Antibody / Lymphoid enhancer-binding factor 1 / TCF1 alpha

Lymphoid Enhancing Factor 1 (LEF1), also called T cell-specific transcription factor 1-alpha (TCF1 alpha) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 2-4 µg/mL

UniProt

Q9UJU2

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 100-200 was used as the immunogen for the LEF1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

LEF1/341R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the LEF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This LEF1 antibody is available for research use only.

Storage Conditions

Aliquot the LEF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the LEF1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human lymph node with LEF1 antibody (clone LEF1/341R) at 2ug/ml in PBS for 30min RT. Strong nuclear staining of non-germinal center observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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