Recombinant HSP27 Antibody / HSPB1
HSP27, also referred to as Heat shock protein beta-1 (HSPB1), Estrogen-Regulated 24K protein, and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P04792
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human full-length HSP27 protein was used as the immunogen for the recombinant HSP27 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
HSPB1/6490R
Type
Recombinant
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant HSP27 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant HSP27 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant HSP27 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant HSP27 antibody should be determined by the researcher.
Location
Predominantly cytoplasmic with some nuclear
Image Legend
Western blot testing of human liver tissue lysate using recombinant HSP27 antibody (clone HSPB1/6490R) . Predicted molecular weight: 23-27 kDa.
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