Recombinant EGF Receptor / EGFR Antibody (Extracellular domain)

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P00533

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 25-645 from the extracellular domain of the human protein were used as the immunogen for the recombinant EGF Receptor antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2a κ

Clone

REGFR/6389

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant EGF Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant EGF Receptor antibody is available for research use only.

Storage Conditions

Aliquot the recombinant EGF Receptor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant EGF Receptor antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human placental tissue with recombinant EGF Receptor antibody (clone rEGFR/6389) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.