Recombinant HSP60 Antibody
Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60) . A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s) . hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P10809
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant full-length human HSPD1 protein was used as the immunogen for the recombinant HSP60 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2a κ
Clone
RHSPD1/6495
Type
Recombinant
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant HSP60 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant HSP60 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant HSP60 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant HSP60 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human Hep2 cell lysate using recombinant HSP60 antibody (clone rHSPD1/6495) . Predicted molecular weight: ~60 kDa.
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