Pancreas/duodenum homeobox protein 1 Antibody / PDX1

Pancreatic duodenal homeobox-1 protein (PDX-1), also designated Insulin promoter factor (IPF1), Insulin upstream factor 1 (IUF1), somatostatin transactivating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic cells. PDX-1 binds to the sequence C (C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis. The gene which encodes PDX-1 maps to human chromosome 13q12.2.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

UniProt

P52945

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human PDX1 protein was used as the immunogen for the Pancreas/duodenum homeobox protein 1 antibody.

Clonality

Monoclonal

Isotype

IgG2c

Clone

PCRP-PDX1-2C11

Applications

FACS, IF

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the Pancreas/duodenum homeobox protein 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Pancreas/duodenum homeobox protein 1 antibody is available for research use only.

Storage Conditions

Aliquot the Pancreas/duodenum homeobox protein 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Pancreas/duodenum homeobox protein 1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Immunofluorescent staining of PFA-fixed human HeLa cells using Pancreas/duodenum homeobox protein 1 antibody (green, clone PCRP-PDX1-2C11) and phalloidin (red) .