RPS6KA5 Antibody / MSK1
The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, have been implicated as important signaling intermediates in response to a broad range of ligand-activated receptor tyrosine kinases. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. A related S6 kinase, p70 S6 kinase, functions to phosphorylate the S6 protein on ribosomal 40S subunits. p70 S6 kinase b shares high sequence homology with p70 S6 kinase, except in the carboxy terminus where it contains a proline-rich domain that may be involved in SH3 domain containing protein interactions. MSK1 (also designated RLPK) is related to Rsk and p70 S6 kinase family members and is thought to be structurally similar to Rsk family members, but it may be regulated by distinct mechanisms.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
O75582
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human RPS6KA5 protein was used as the immunogen for the RPS6KA5 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
PCRP-RPS6KA5-1A8
Applications
FACS, IF, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the RPS6KA5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This RPS6KA5 antibody is available for research use only.
Storage Conditions
Aliquot the RPS6KA5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the RPS6KA5 antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm
Image Legend
Immunofluorescent staining of human HeLa cells using RPS6KA5 antibody (green, clone PCRP-RPS6KA5-1A8) and phalloidin (red) .
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