UBE2B Antibody
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (ubiquitin-conjugating enzyme E2 A) and UBE2B (ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to post replication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
P63146
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human UBE2B protein was used as the immunogen for the UBE2B antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
PCRP-UBE2B-1C7
Applications
FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the UBE2B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This UBE2B antibody is available for research use only.
Storage Conditions
Aliquot the UBE2B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the UBE2B antibody should be determined by the researcher.
Location
Cell membrane, Nucleus
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells using UBE2B antibody (green, clone PCRP-UBE2B-1C7) and phalloidin (red) .
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