ZFP64 Antibody

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Kr ppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZFP64 (Zinc finger protein 64), also known as ZNF338, is a 681 amino acid homolog of the mouse Zfp64 protein and is a member of the Kr ppel C2H2-type zinc-finger family. Localized to the nucleus, ZFP64 contains nine C2H2-type zinc fingers and is thought to be involved in transcriptional regulation. Four isoforms of ZFP64 exist due to alternative splicing events.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q9NTW7

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human ZFP64 protein was used as the immunogen for the ZFP64 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-ZFP64-1H2

Applications

FACS, IF, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the ZFP64 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ZFP64 antibody is available for research use only.

Storage Conditions

Aliquot the ZFP64 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the ZFP64 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm, Cell surface

Image Legend

IHC staining of FFPE human lymph node tissue with ZFP64 antibody (clone PCRP-ZFP64-1H2) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.