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SMAD3 Antibody

Smad proteins, the mammalian homologs of the Drosophila mothers against dpp (Mad) have been implicated as downstream effectors of TGF-mediated phosphorylation of other Smad family members.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

UniProt

P84022

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human SMAD3 protein was used as the immunogen for the SMAD3 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

PCRP-SMAD3-1A2

Applications

FACS, IF

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SMAD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SMAD3 antibody is available for research use only.

Storage Conditions

Aliquot the SMAD3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SMAD3 antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

Immunofluorescent staining of PFA-fixed human HeLa cells using SMAD3 antibody (green, clone PCRP-SMAD3-1A2) and phalloidin (red) .

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