SMAD3 Antibody
Smad proteins, the mammalian homologs of the Drosophila mothers against dpp (Mad) have been implicated as downstream effectors of TGF-mediated phosphorylation of other Smad family members.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
P84022
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human SMAD3 protein was used as the immunogen for the SMAD3 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
PCRP-SMAD3-1A2
Applications
FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the SMAD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SMAD3 antibody is available for research use only.
Storage Conditions
Aliquot the SMAD3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the SMAD3 antibody should be determined by the researcher.
Location
Cytoplasm, Nucleus
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells using SMAD3 antibody (green, clone PCRP-SMAD3-1A2) and phalloidin (red) .
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