Sirtuin 2 Antibody / SIRT2
The silent information regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in a NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT proteins may function via mono-ADP-ribosylation of proteins. SIRT2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
Q8IXJ6
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human SIRT2/Sirtuin 2 protein was used as the immunogen for the Sirtuin 2 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
PCRP-SIRT2-1A8
Applications
FACS, IF, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Sirtuin 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Sirtuin 2 antibody is available for research use only.
Storage Conditions
Aliquot the Sirtuin 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Sirtuin 2 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells using Sirtuin 2 antibody (green, clone PCRP-SIRT2-1A8) and phalloidin (red) .
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