SPIC Antibody / Transcription factor Spi-C

The Ets transcription factor family is comprised of DNA-binding proteins that influence lymphoid development and activity and bind the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the Ets domain. Spi-B and Spi-C (also known as SPIC) are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to non-canonical AGAA sites. Spi-C is a 248 amino acid protein that localizes to the nucleus and, like other Ets family members, binds DNA as a monomer and plays a role in transcriptional regulation. Addi- tionally, Spi-C is thought to control the development of red pulp macrophages, thereby contributing to iron homeostasis and red blood cell recycling. Human Spi-C shares 65% amino acid identity with its mouse counterpart, suggesting a conserved role between species.

Product Specifications

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

UniProt

Q8N5J4

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human Transcription factor Spi-C protein was used as the immunogen for the SPIC antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-SPIC-2C5

Applications

FACS, IF

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the SPIC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SPIC antibody is available for research use only.

Storage Conditions

Aliquot the SPIC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the SPIC antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using SPIC antibody (clone PCRP-SPIC-2C5) . These results demonstrate the foremost specificity of the PCRP-SPIC-2C5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.