SMNDC1 Antibody

SPF30 (survival of motor neuron-related-splicing factor 30), also known as SMNDC1 (survival motor neuron domain containing 1) or SMNR (SMN-related protein), is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (survival motor neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. SPF30 plays an important role in spliceosome assembly and directly interacts with five U snRNPs. The loss of SPF30 causes spliceosome assembly to arrest at prespliceosomes (A complex) . This supports a function for SPF30 in mediating the incorporation/recruitment of U4/U5/U6 tri-snRNP to the prespliceosome. In addition, the overexpression of SPF30 can lead to apoptosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

UniProt

O75940

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human SMNDC1 protein was used as the immunogen for the SMNDC1 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-SMNDC1-1A9

Applications

FACS, IF

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SMNDC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SMNDC1 antibody is available for research use only.

Storage Conditions

Aliquot the SMNDC1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SMNDC1 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using SMNDC1 antibody (clone PCRP-SMNDC1-1A9) . These results demonstrate the foremost specificity of the PCRP-SMNDC1-1A9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.