Phospho-Histone H3 Antibody (pS10)
Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%) . Increased expression of PHH3 was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clarks level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002) . For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P68431
Host
Mouse
Reactivity
Human
Immunogen
Amino acids ARK (pS) TGGKAPRKQLc) were used as the immunogen for the phospho-Histone H3 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RPHH3/6824
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the phospho-Histone H3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-Histone H3 antibody is available for research use only.
Storage Conditions
Aliquot the phospho-Histone H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/phospho-histone-h3-antibody-ps10-rphh36824-v9213
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the phospho-Histone H3 antibody should be determined by the researcher.
Location
Nucleus, Chromosome
Image Legend
Western blot testing of human MCF7 cell lysate with phospho-Histone H3 antibody.
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