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DDX41 Antibody

DDX41 (Probable ATP-dependent RNA helicase DDX41, DEAD box protein abstrakt homolog) is a 622 amino acid protein encoded by the human gene DDX41. DDX41 belongs to the DEAD box helicase family (DDX41 subfamily) and contains one CCHC-type zinc finger, one helicase ATP-binding domain and one helicase C-terminal domain. DDX41 is required during post-transcriptional gene expression and is thought to be involved in pre-mRNA splicing. DDX41 is believed to be a probable ATP-dependent RNA helicase. RNA helicases are highly conserved enzymes that utilize the energy derived from NTP hydrolysis to modulate the structure of RNA. RNA helicases participate in all biological processes that involve RNA, including transcription, splicing and translation.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL

UniProt

Q9UJV9

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human DDX41 protein was used as the immunogen for the DDX41 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-DDX41-1B4

Applications

FACS, WB, IF

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the DDX41 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This DDX41 antibody is available for research use only.

Storage Conditions

Aliquot the DDX41 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/ddx41-antibody-pcrp-ddx41-1b4-v9206

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the DDX41 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using DDX41 antibody (clone PCRP-DDX41-1B4) . These results demonstrate the foremost specificity of the PCRP-DDX41-1B4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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