IRF3 Antibody / Interferon regulatory factor 3
In the absence of viral infection, IRF3 is maintained as a monomer in an autoinhibited state. Phosphorylation by TBK1 and IKBKE disrupts this autoinhibition leading to the liberation of the DNA-binding and dimerization activities and its nuclear localization where it can activate type I IFN and ISG genes. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
Q14653
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human IRF3 protein was used as the immunogen for the IRF3 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
PCRP-IRF3-6C8
Applications
FACS, IF, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the IRF3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This IRF3 antibody is available for research use only.
Storage Conditions
Aliquot the IRF3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the IRF3 antibody should be determined by the researcher.
Location
Cytoplasm, Nucleus
Image Legend
Immunofluorescent staining of human MCF-7 cells using IRF3 antibody (green, clone PCRP-IRF3-6C8) and phalloidin (red) .
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