ICAM1 Antibody / CD54

ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P05362

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human ICAM1 protein was used as the immunogen for the ICAM1 antibody. The epitope is is known to be in the extracellular domain.

Clonality

Monoclonal

Isotype

IgG1

Clone

ICAM1/6319

Applications

FACS, IF, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the ICAM1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ICAM1 antibody is available for research use only.

Storage Conditions

Aliquot the ICAM1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the ICAM1 antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human tonsil tissue with ICAM1 antibody (clone ICAM1/6319) at 2ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.