Eukaryotic translation initiation factor 4E Antibody / EIF4E

The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA cap during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

P06730

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human EIF4E/Eukaryotic translation initiation factor 4E protein was used as the immunogen for the Eukaryotic translation initiation factor 4E antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

PCRP-EIF4E-1D3

Applications

FACS, IF, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Eukaryotic translation initiation factor 4E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Eukaryotic translation initiation factor 4E antibody is available for research use only.

Storage Conditions

Aliquot the Eukaryotic translation initiation factor 4E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Eukaryotic translation initiation factor 4E antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunofluorescent staining of PFA-fixed human MCF-7 cells using Eukaryotic translation initiation factor 4E antibody (green, clone PCRP-EIF4E-1D3) and phalloidin (red) .