EIF4A2 Antibody

Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a5 cap, eIF4A (I, II) are bidirectional RNA helicases, and eIF4G (I, II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating)

UniProt

Q14240

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human EIF4A2 protein was used as the immunogen for the EIF4A2 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

PCRP-EIF4A2-2B5

Applications

ELISA

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the EIF4A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This EIF4A2 antibody is available for research use only.

Storage Conditions

Aliquot the EIF4A2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the EIF4A2 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using EIF4A2 antibody (clone PCRP-EIF4A2-2B5) . These results demonstrate the foremost specificity of the PCRP-EIF4A2-2B5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.