EIF4A2 Antibody
Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a5 cap, eIF4A (I, II) are bidirectional RNA helicases, and eIF4G (I, II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating)
UniProt
Q14240
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human EIF4A2 protein was used as the immunogen for the EIF4A2 antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
PCRP-EIF4A2-2B5
Applications
ELISA
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the EIF4A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This EIF4A2 antibody is available for research use only.
Storage Conditions
Aliquot the EIF4A2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/eif4a2-antibody-pcrp-eif4a2-2b5-v9191
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the EIF4A2 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
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