E4F1 Antibody
E4F1 (E4F transcription factor 1), also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains 9 C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
Q66K89
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human E4F1 protein was used as the immunogen for the E4F1 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
PCRP-E4F1-2D1
Applications
FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the E4F1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This E4F1 antibody is available for research use only.
Storage Conditions
Aliquot the E4F1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the E4F1 antibody should be determined by the researcher.
Location
Nucleus, cytoplasm
Image Legend
Immunofluorescent staining of PFA-fixed MCF7 cells using E4F1 antibody (green, clone PCRP-E4F1-2D1) and phalloidin (red) .
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