Daxx Antibody

Daxx interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, it functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

UniProt

Q9UER7

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human DAXX protein was used as the immunogen for the Daxx antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

PCRP-DAXX-8B7

Applications

FACS, IF

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Daxx antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Daxx antibody is available for research use only.

Storage Conditions

Aliquot the Daxx antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Daxx antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using Daxx antibody (clone PCRP-DAXX-8B7) . These results demonstrate the foremost specificity of the PCRP-DAXX-8B7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.