Recombinant CCNE1 Antibody / Cyclin E1
Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
IDP24864
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 10-176 was used as the immunogen for the recombinant CCNE1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
CCNE1/4935R
Type
Recombinant
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant CCNE1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant CCNE1 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant CCNE1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CCNE1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of human HCT-116 cell lysate using recombinant CCNE1 antibody (clone CCNE1/4935R) . Predicted molecular weight ~47 kDa.
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