Recombinant WT1 Antibody / Wilms Tumor 1
The WT1 gene located at chromosome 11p13 codes for a transcription factor, a DNA-binding nucleoprotein, that plays a role primarily in the development of genitourinary organs. There are at least eight isoforms ranging between 52 and 62kDa produced by a combination of alternative splicing and RNA editing. WT1 is synthesized and reside in the cytoplasm in an inactive form. When activated through phosphorylation it is translocated to the nucleus. In tumor tissues, WT1 is detected in tumor cells of Wilms Tumor (also known as nephroblastoma) and mesothelioma. Additionally, WT1 expression has been found in ovarian serous carcinomas and some breast carcinomas. WT1 is particularly used for distinguishing malignant mesothelioma and ovarian serous carcinoma from non-serous carcinomas.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P19544
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 1-100 was used as the immunogen for the recombinant WT1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RWT1/6908
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant WT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant WT1 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant WT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant WT1 antibody should be determined by the researcher.
Location
Nucleus and cytoplasm
Image Legend
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