Recombinant CDH1 Antibody / E-Cadherin

E-cadherin/Cadherin-1/CDH1 is a transmembrane, calcium dependent cell adhesion protein that mediates cell to cell adhesion and maintains structural and functional integrity of epithelial tissues.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

UniProt

P12830

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 600-700 of E-cadherin protein was used as the immunogen for the recombinant CDH1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CDH1/7034R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant CDH1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant CDH1 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant CDH1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant CDH1 antibody should be determined by the researcher.

Location

Cytoplasmic, membranous

Image Legend

IHC staining of FFPE human colon tissue with recombinant CDH1 antibody (clone CDH1/7034R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.