Recombinant p27Kip1 Antibody / p27

P27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-beta-induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P46527

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human CDKN1B/p27Kip1 protein was used as the immunogen for the recombinant p27Kip1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RKIP1/1356

Type

Recombinant

Applications

FACS, IF, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant p27Kip1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant p27Kip1 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant p27Kip1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant p27Kip1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human breast carcinoma tissue with recombinant p27Kip1 antibody (clone rKIP1/1356) . Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.