Recombinant SYP Antibody / Synaptophysin

This Monoclonal antibody recognizes a protein of 38kDa that is identified as Synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 2-4 µg/mL

UniProt

P08247

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 224-313 was used as the immunogen for the recombinant SYP antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RSYP/6856

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant SYP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant SYP antibody is available for research use only.

Storage Conditions

Aliquot the recombinant SYP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant SYP antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human brain tissue with recombinant SYP antibody (clone rSYP/6856) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.