Recombinant Cyclin E1 Antibody / CCNE1
Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
IDP24864
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 10-176 was used as the immunogen for the Cyclin E1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RCCNE1/4936
Type
Recombinant
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant Cyclin E1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant Cyclin E1 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant Cyclin E1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Cyclin E1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of human HCT116 cell lysate using recombinant Cyclin E1 antibody (clone rCCNE1/4936) . Predicted molecular weight ~47 kDa.
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