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Anti-BrdU Antibody

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

Not Applicable

Host

Mouse

Reactivity

All Species Expected

Immunogen

Bromodeoxyuridine was used as the immunogen for this anti-BrdU antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SPM537

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the anti-BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This anti-BrdU antibody is available for research use only.

Storage Conditions

Store the anti-BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Nuclear

Image Legend

IHC: Formalin-fixed, paraffin-embedded mouse small intestine stained with anti-BrdU antibody (SPM537) .

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