Anti-Golgi Antibody
This mAb recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Not Known
Host
Mouse
Reactivity
Human
Immunogen
SU-DHL-1 large cell lymphoma cells were used as the immunogen for this anti-Golgi antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM581
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the anti-Golgi antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This anti-Golgi antibody is available for research use only.
Storage Conditions
Store the anti-Golgi antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Golgi complex in cytoplasm
Image Legend
IHC: Formalin-fixed, paraffin-embedded human gallbladder stained with anti-Golgi antibody (SPM581) .
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