Anti-CD44 Antibody
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes. Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s) . Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT
UniProt
P16070
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for this anti-CD44 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
SPM544
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the anti-CD44 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This anti-CD44 antibody is available for research use only.
Storage Conditions
Store the anti-CD44 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface, cytoplasmic
Image Legend
Western blot testing of human HeLa cell lysate with anti-CD44 antibody (clone SPM544) . Predicted molecular weight ~81 kDa.
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